Waters HPLC Diagnóstico Y Corrección De Anomalías página 115

Problem – – Sample Draw Volume Problem
Reproducibility - - Reproducibility
Symptom:
to injection from the same vial
— Possible
draw port needle,
septum material
o
— Solution:
o
o
o
•Silicone Septum
o
•Material Lodges
in Bottom Draw
Needle
Sample Draw Volume
Coring of Septum Coring of Septum
Peak area varies (increases /decreases) from injection
cause:Coring of Septum by Needle. If using a
the draw port could be plugged with
Check needle draw port for septum material, remove /
replace.
Should self sealing PTFE/silicone septum be used:
Switch to pre-slit PTFE/silicone septum
Pre-slit septum will eliminate coring and deliver good
resealing capability
"Alternatively"
Switch to PTFE septum
PTFE will eliminate coring. However, it will not reseal
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