Waters HPLC Diagnóstico Y Corrección De Anomalías página 69

Impact On Mobile Phase pH on Impact On Mobile Phase pH on
Retention Factor (k) Reproducibility Retention Factor (k) Reproducibility
•100
•Acid
•10
•(Un-ionized)
•1
•Base
1+2 1+2
•0.1
•0 •2
•(Ionized)
•B2
•pH 5.5
•B1
•N
•pH: Powerful tool in methods development for Selectivity –
Range where pH control will be critical
•4 •6
•A
•Base 2
•Un-ionized
•Base 1
•Neutral
•pH
•8 •10 •12
•N
•A, B1
•pH6.0
•Acid
(Ionized) (Ionized)
•14
•B2
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