Waters HPLC Diagnóstico Y Corrección De Anomalías página 59

Effect of solvent composition Effect of solvent composition
(% organic) RP chromatography (% organic) RP chromatography
Exponential Relationship
between k and Volume %
Organic
Retention Time Change
of 5% to 15% per 1% of 5% to 15% per 1%
Change in Solvent
Composition
Bonded Phase Collapse in
High Water Content
(discussed later)
Retention Tim es vs. % Methanol
35.00
30.00 30.00
25.00
20.00
15.00
10.00
5.00
0.00
35 45
% Methanol
55 65
©2007 Waters Corporation 59