Waters HPLC Diagnóstico Y Corrección De Anomalías página 45

Column/Volume Overload Column/Volume Overload
6.25 g injected in 5 L
% of column volume: 0.625
2
6.25 g injected in 500 L
% of column volume: 62.5
2
4
Minutes
4
Minutes
Column Volume:
0.8 mL (800 L)
(4.6 X 50mm ODS)
Wider peaks first Wider peaks first
observed at low
6
retention
( )
Peak position shifts
to higher retention
in proportion to the
injection volume
( )
6
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