Waters HPLC Diagnóstico Y Corrección De Anomalías página 72

Retention Time Reproducibility
Ion Pairing Reagents
(E.g. alkyl sulphonates / tetra alkyl ammonium salts)
Retention
Increases Proportional to the Concentration of the
–
Pairing Agent at Low Concentration Pairing Agent at Low Concentration
Nearly Independent of the Concentration of the
–
Pairing Agent at High
Long Equilibration Times
Due to Adsorption of the Reagent on the Stationary
–
Phase (can be up to 500 Column Volumes)
(~10mM/L) Concentration
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