Waters HPLC Diagnóstico Y Corrección De Anomalías página 91

Tips from Case Studies Tips from Case Studies
When replacing a column that has been used for
many injections you should expect that the surface
has been modified to some degree
The new column will/may give different results
initially (especially if the ligand was hydrolyzed off the
old column) old column)
The new column will have more ligand, and therefore
changed retention characteristics.
•During Methods Development, running a "used"
•can sometimes show degree of robustness
column
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