Waters HPLC Diagnóstico Y Corrección De Anomalías página 62

Mobile Phase Composition Mobile Phase Composition
is Critical for Reproducibility is Critical for Reproducibility
•Options A and A'
•A) 600 mL
•A') 6?? mL
• • 1000 mL 1000 mL
•Options A and A': Adding organic/aqueous directly to measuring vessel
• Need to specify which solvent is added first: e.g. H2O to MeOH or MeOH to H
•
•
•Standardize pH Adjustment – ie; BEFORE organic is added
•60/40 (MeOH/Water)
•??? mL
•400 mL
•Difference due to
•partial volumes*
•*
•Note: Partial Volumes on mixing may give
• more or less than initial volumes
•Options B/C
• • 600 mL 600 mL
• • • • abc gr abc gr abc gr abc gr
•* 960mL
(not recommended)
• • 400 mL 400 mL
• • xyz gr xyz gr
•*
• • <1000 mL <1000 mL
O
2
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