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Waters HPLC Diagnóstico Y Corrección De Anomalías página 127

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Column Regeneration
Column Regeneration
Always follow column vendor's guideline for
regeneration
Regeneration can bring back a column's performance
if problem relates to compounds, which are retained
under method conditions, causing changes in
under method conditions, causing changes in
chromatography. Washing them off with more
aggressive solvents can return performance
If surface has been chemically altered, ie hydrolysis of
ligands, then performance may not be restored
©2007 Waters Corporation
127

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