Waters HPLC Diagnóstico Y Corrección De Anomalías página 18

Performance of Different Column Diameters Performance of Different Column Diameters
with Normal Instrument Band Spreading (80 L) with Normal Instrument Band Spreading (80 L)
10000
4.6mm ID
9000
8000
7000
6000 6000
5000
4000
3000
2000
1000
0
0 1
All Columns "would"
provide 10,000 plates --
Impact greatest for early eluters
3.9mm ID
3.0mm ID
2.0 mm ID 2.0 mm ID
2 3 4 5 6 7
Retention Factor
(k)
Peak Width due to
Instrument Band Spreading
exceeding Column Band
Spreading
1.0 mm ID
8 9 10 11 12 13 14 15 16 17 18 19 20
Note: You will not get optimum plate
count performance on standard
instruments as you reduce the column ID,
especially below 2.0mm ID
4.6 4.6
3.9
3.0
2.0
1.0
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