Waters HPLC Diagnóstico Y Corrección De Anomalías página 128

Column Regeneration/Cleaning Column Regeneration/Cleaning
Flush with 20 column volumes. Run columns in reverse flow direction,
disconnected from detector. Cleaning efficiency is increased at 35-45ºC
Reversed phase
columns:
Methanol*
THF
Hexane
THF
Methanol
Mobile phase*
•
•
•* Make sure mobile phase is miscible w. methanol and no salt precipitation occurs
Normal phase columns:
50/50
Methanol/chloroform
Ethyl acetate
Mobile phase
•
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