Waters HPLC Diagnóstico Y Corrección De Anomalías página 79

Re- - wetting a Stationary Phase Re wetting a Stationary Phase
Once De- - wetted: Once De wetted:
Use a mobile phase containing > 40 % methanol or
other polar organic solvent
Difficult to use pressure to force aqueous mobile Difficult to use pressure to force aqueous mobile
phase back into pores
Typically not practical because column outlet is at atmospheric pressure
•Inlet
•2200 psi
•P
•Length
•Outlet
•1 atm (14.7 psi)
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