Waters HPLC Diagnóstico Y Corrección De Anomalías página 84

•Changes in Retention/Resolution Method
•Once the column is altered in your laboratory,
if you use this "modified particle surface" to
create a separation, you may not be able to
reproduce that separation on a new column – reproduce that separation on a new column –
because the new column was not chemically
•Methods Development Risk – starting with a "used column"
•(in a drawer, on the instrument, donated by a colleague)
•"Regeneration" may not always bring you back to the original
chromatographic surface, especially, if ligand hydrolysis (loss of ligand) or
surface modification by ion-pairing reagents has occurred
Development
altered in the same way.
©2007 Waters Corporation 84