Waters HPLC Diagnóstico Y Corrección De Anomalías página 130

Column Regeneration/Cleaning Column Regeneration/Cleaning
•If you suspect contamination with proteins :
Inject 100 µl DMSO while flushing the column with
100% acetonitrile
Run a 10-100% B gradient where
A= 0.1% TFA in water A= 0.1% TFA in water
B= 0.1% TFA in acetonitrile
Flush with 7M guanidine HCl or 7M urea
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