Waters HPLC Diagnóstico Y Corrección De Anomalías página 14

Band spreading Band spreading
How do you get sharp peaks
with
— Well Shaped
— (Good Mechanical And Chemical Performance)
Isocratic Isocratic
ELUTING FROM THE COLUMN
Yellow Yellow analyte: narrowest band width – sharpest peak
Red analyte: moderate band width – moderate peak width
Blue analyte: Slowest – BROADEST/MOST DILUTED BAND --
Broadest Peak (longer on the column – takes more mobile phase
to sweep it out of the column -- broader the peak)
excellent
Bands -- Well Separated
Detector Cell Detector Cell
resolution?
Waters c 1998
©2007 Waters Corporation 14