Waters HPLC Diagnóstico Y Corrección De Anomalías página 77

How does flow stoppage cause this problem How does flow stoppage cause this problem
with an HPLC column? with an HPLC column?
•Analytes
•Analytes
•Stopped flow with no pressure on the
•At flow with pressure on the
mobile phase, pores de-wet. Restart flow,
mobile phase.
pores remain de-wetted with analytes
never entering pores (low surface area).
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